Coding

Part:BBa_K2476014:Design

Designed by: Vivian Hu   Group: iGEM17_Pittsburgh   (2017-10-27)


Mutant CheY + Linker/Dronpa/Linker at 60-61


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 193
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 142
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for Escherichia coli K12 and checked for BioBrick restriction sites using IDT Codon Optimization Tool.

Mutant chemotaxis protein CheY with flexible serine-glycine linkers and a Dronpa monomer attached between M60-P61.

Source

Constitutively active double mutant CheY D13K Y106W with flexible serine-glycine linkers and a pdDronpa1.2 monomer (http://science.sciencemag.org/content/355/6327/836.full) attached between M60-P61. Synthesized by IDT.

References